Detection of Clonal T-Cell Receptor-gamma Gene Rearrangement by PCR/Temporal Temperature Gradient Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Cutaneous T-cell infiltrates: analysis of T-cell receptor gamma gene rearrangement by polymerase chain reaction and denaturing gradient gel electrophoresis.
In cutaneous T-cell infiltrates, the demonstration of a clonal T-cell receptor (TCR) gene rearrangement has been considered helpful to distinguish Cutaneous T-cell lymphomas from reactive lymphoproliferation. Hence, a polymerase chain reaction (PCR) method using GC-clamp primers and denaturing gradient gel electrophoresis has been developed in our laboratory to analyze the TCR gamma locus confi...
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INTRODUCTION Polymerase chain reaction (PCR)-based molecular techniques are useful adjunctive tools in the diagnosis of cutaneous T-cell lymphomas (CTCL). This study compares the sensitivity of PCR analysis of the T-cell receptor-gamma (TCR-gamma) gene rearrangements using conventional polyacrylamide gel electrophoresis (PCR-PAGE) and fluorescent capillary electrophoresis (PCR-FCE). MATERIALS...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2001
ISSN: 0002-9173,1943-7722
DOI: 10.1309/8kmv-t6bm-g9pc-ek24